| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | BISHOP SNYDER |
A | Carpenter | Jones | Turner | Sparks | NT |
| 3 | RIDGEVIEW (OP) |
A | Marshall | Mess | Zeigler | Willis | 3:42.43 |
| 4 | ORANGE PARK |
A | Richardson | Riley | Bradley | Campbell | 3:36.21 |
| 5 | CLAY |
A | Weiss | Pierre | Looney | Baillie | NT |
| 6 | MENENDEZ |
A | Johnson | Smith | Sivyer | Korfhage | 3:44.84 |
| 7 | MIDDLEBURG |
A | Wolosuk | Green | Seward-Smith | Araujo | 3:50.88 |