| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | MENENDEZ |
A | Heise | Boss | Ammann | Ziecheck | NT |
| 3 | BISHOP SNYDER |
A | Bawroski | Tran | Greene | McInnis | NT |
| 4 | ORANGE PARK |
A | Ambrose | Davis | Porter | Henry | 4:14.52 |
| 5 | RIDGEVIEW (OP) |
A | Benda | Silva | Burnett | Saint-vil | 4:38.35 |
| 6 | MIDDLEBURG |
A | Mcgriff | Delia | King | Chaon | 4:56.67 |