| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | BISHOP SNYDER |
A | Bing | Preston | Walker | Bawroski | NT |
| 3 | MIDDLEBURG |
A | Mckenzie | Herren | Wesley | Ornelas | 52.90 |
| 4 | ORANGE PARK |
A | Young | Brown | Pacheco | Porter | 50.86 |
| 5 | RIDGEVIEW (OP) |
A | Lee | Dachoute | Taravine | Miller | 52.23 |
| 6 | BRADFORD |
A | Booker | Fergerson | Shanks | NT | |
| 7 | MENENDEZ |
A | Smith | Johnson | Gooden | Lagana | NT |