| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 |  BISHOP SNYDER | A | Bing | Preston | Walker | Bawroski | NT | 
| 3 |  MIDDLEBURG | A | Mckenzie | Herren | Wesley | Ornelas | 52.90 | 
| 4 |  ORANGE PARK | A | Young | Brown | Pacheco | Porter | 50.86 | 
| 5 | .png) RIDGEVIEW (OP) | A | Lee | Dachoute | Taravine | Miller | 52.23 | 
| 6 |  BRADFORD | A | Booker | Fergerson | Shanks | NT | |
| 7 |  MENENDEZ | A | Smith | Johnson | Gooden | Lagana | NT |