| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | LAKE PLACID |
A | Williams | Felton, Jr. | Bullard, Jr. | Johnson | 44.46 |
| 3 | HARDEE |
A | Delhomme | Myrick | Mullins | Cherry | 43.65 |
| 4 | SEBRING |
A | Walker | Hamilton | Peterson | Austin | 43.78 |
| 5 | DESOTO COUNTY |
A | Bettercherly | Kinville | Ames | Quezada | 45.65 |