| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | MIDDLEBURG |
A | Mcgriff | Glidwell | Toomey | Delia | NT |
| 3 | TRINITY CHRISTIAN (JAX) |
A | Johnson | Yarborough | Landreth | Graham | NT |
| 4 | BISHOP SNYDER |
A | Bawroski | McInnis | Greene | Tran | 4:43.04 |
| 5 | ST. JOSEPH |
A | Revels | Andrews | Doctora | Clare | NT |
| 6 | DUVAL CHARTER BAYMEADOWS |
A | Halili | Emmanuel | olajide | Torres | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | MANDARIN |
A | Williams | Togbui | Wyche | Jolly | NT |
| 2 | BARTRAM TRAIL |
A | Mullis | Williams | Braughton | Dison | 4:14.00 |
| 3 | FLEMING ISLAND |
A | Steeves | Wonpat | Mota | Smith | NT |
| 4 | OAKLEAF |
A | Gaines | Ledgister | Evans | Lawson | 4:04.15 |
| 5 | ORANGE PARK |
A | Ambrose | Mazerac | Young | Henry | 4:11.84 |
| 6 | PALATKA |
A | Watts | Roberts | Passmore | Dean | NT |
| 7 | ST. JOHNS COUNTRY DAY |
A | Barry | Williams | Schmidt | Sunderhaus | NT |
| 8 | RIDGEVIEW (OP) |
A | Benda | Burnett | Silva | Rios | 4:42.80 |