| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | BARTRAM TRAIL |
A | McLean | May | Renner | Hyman | 11:00.00 |
| 2 | TRINITY CHRISTIAN (JAX) |
A | Barley | Seiler | Yarborough | Landreth | 11:45.00 |
| 3 | RIDGEVIEW (OP) |
A | Burnett | Silva | Turner | Thomas | 11:10.96 |
| 4 | CHRISTS CHURCH ACADEMY |
A | Love | Smith | Quinn | Raulerson | NT |
| 5 | ST. JOHNS COUNTRY DAY |
A | Stratton | Robinson | Sunderhaus | Staten | 11:27.00 |
| 6 | ORANGE PARK |
A | Mazerac | Wilson | Mazerac | Wages | NT |
| 7 | FLEMING ISLAND |
A | Bertolini | Cornell | Gillaspy | Fallin | 9:30.66 |
| 8 | PALATKA |
A | Dean | Frisvold | Passmore | Watts | NT |
| 9 | OAKLEAF |
A | Camacho | Cameron | Evans | Smith | 10:56.93 |
| 10 | ST. AUGUSTINE |
A | Gates | Conroy | Flaherty | Rios | 10:10.00 |
| 11 | BISHOP SNYDER |
A | Bawroski | Brooks | Cardozo | McInnis | 11:46.61 |
| 12 | CLAY |
A | Knabb | Martinez | Mello | Williamson | NT |
| 13 | ST. JOSEPH |
A | Castiglia | Haverly | Morin | Bennett | NT |