| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | FERNANDINA BEACH |
A | Jordan | Maldonado-Eljaiek | Miller | Kallstrand | 45.50 |
| 2 | MIDDLEBURG |
A | Araujo | Boyd | Padgett | Gilbert | 45.00 |
| 3 | BARTRAM TRAIL |
A | Dawkins | Robinson | Brown | Carvalho | 44.47 |
| 4 | RIDGEVIEW (OP) |
A | Felder | Evans | Watkins | Marshall | NT |
| 5 | BISHOP SNYDER |
A | Carney | Jones | Navitsky | Mitchell | 44.45 |
| 6 | TRINITY CHRISTIAN (JAX) |
A | Tre | Sterdivant | Hawkins | Cook | NT |
| 7 | FLORIDA DEAF AND BLIND |
A | Bell | Hoffnagle | Valcin | Allen | 45.18 |
| 8 | DUVAL CHARTER BAYMEADOWS |
A | Napolitan | Letourneau | Winchester | Smith | 45.89 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | CLAY |
A | Addison | Burke | Blaha | Thomas | NT |
| 2 | PALATKA |
A | Wright Jr | Turner | Valentine | Barnes | 43.38 |
| 3 | ORANGE PARK |
A | Crockett II | Campbell | Verger | Richardson | 42.03 |
| 4 | OAKLEAF |
A | Davis | Collins | Warthen | Brown | 41.41 |
| 5 | FLEMING ISLAND |
A | Burney | Cruz | Broughton | Singleton | 41.98 |
| 6 | MANDARIN |
A | Lane | Montlouis | Summerall | Wyche | 42.90 |
| 7 | ST. AUGUSTINE |
A | Anderson | Major | James | Jones | 43.38 |
| 8 | FLAGLER PALM COAST |
A | Jacobelli | Haymon | Batson | Ajede | NT |