| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | FLORIDA DEAF AND BLIND |
A | Kramer | Haggerty | Abenchuchan | Swartzlander | 9:11.62 |
| 2 | FLAGLER PALM COAST |
A | Phok | Murray | Olsen | Johnson | 8:15.02 |
| 3 | ST. JOHNS COUNTRY DAY |
A | Graham | Kistner | Nichols | Robinson | 9:45.00 |
| 4 | ST. AUGUSTINE |
A | Dougherty | Lowes | Phelps | Washburn | 8:36.38 |
| 5 | MIDDLEBURG |
A | Millis | Shaw | Kara | Poppell | 8:41.49 |
| 6 | BISHOP SNYDER |
A | Sparks | Carpenter | Fredland | Trinca | 9:08.25 |
| 7 | CLAY |
A | Jewell | Sanders | Luke | Looney | 9:07.46 |
| 8 | FLEMING ISLAND |
A | Middleton | Pugh | Atzert | DeRousse | 7:53.32 |
| 9 | OAKLEAF |
A | Harewood | Memrick | Chester | Swain | 8:50.65 |
| 10 | TRINITY CHRISTIAN (JAX) |
A | Nobles | Brown | Warden | Dukes | 9:34.56 |
| 11 | PALATKA |
A | Gillon | Lugo | McGinnis | Walker | 9:36.11 |
| 12 | RIDGEVIEW (OP) |
A | Mess | Salazar | Montoya | Matz | 8:35.84 |
| 13 | MANDARIN |
A | McLaren | Brugmann | Chattin | Ritz | 10:00.00 |
| 14 | FERNANDINA BEACH |
A | D'Amico | Janzow | Wetherhill | DeVaul | 10:10.50 |
| 15 | BAKER COUNTY |
A | Winkler | Hodges | Bleich | Manalo | NT |