| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | ST. JOSEPH |
A | Morin | Fritis | Hurley | Kramp | NT |
| 2 | MIDDLEBURG |
A | Toomey | Herren | Mckenzie | Ornelas | NT |
| 3 | BAKER COUNTY |
A | Glover | Lofton | Bell | Holley | 57.00 |
| 4 | DUVAL CHARTER BAYMEADOWS |
A | Anderson | Ventress | Hodges | Dean | 53.89 |
| 5 | ST. JOHNS COUNTRY DAY |
A | Williams | Schmidt | Kuczkowski | De Leon | 54.40 |
| 6 | FERNANDINA BEACH |
A | Freeman | Harris | Roberts | Phuong | 59.51 |
| 7 | ST. AUGUSTINE |
A | Grupee | Wright | Wilson | Romulus | DQ |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | RIDGEVIEW (OP) |
A | Lee | Taravine | Dachoute | Miller | 52.20 |
| 2 | BARTRAM TRAIL |
A | McCormick | Thomas | Braughton | Ganther | 50.50 |
| 3 | PALATKA |
A | Andrews | Davis | Fields | Kemp | 50.14 |
| 4 | OAKLEAF |
A | Lawson | Soto | Gaines | McMillian | 48.03 |
| 5 | FLEMING ISLAND |
A | Berkley | Flores | Santana | Shavers | 49.77 |
| 6 | ORANGE PARK |
A | Young | Porter | Pacheco | Brown | 50.29 |
| 7 | CLAY |
A | Aranda | Parks | Reese | Willis | 51.41 |
| 8 | TRINITY CHRISTIAN (JAX) |
A | Galmore | Taylor | Johnson | Rasher | 53.19 |