| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | MENENDEZ |
B | Plumlee | Harris | Raines | Andrews | NT | |
| 2 | MENENDEZ |
A | Oxendine | Smith | Moore | Arroyo | 51.11 | FHSAA 3A Region 1 |
| 3 | FLORIDA DEAF AND BLIND |
A | LeFors | Merchant | Vilsaint | Dize | 54.78 | Palatka Home Meet 2 |
| 4 | PALATKA |
A | Passmore | Williams | Fountain | Oxendine | NT |