| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | MENENDEZ |
A | Martin | Plumlee | Grosso Garcia | Raines | NT | |
| 3 | INTERLACHEN |
A | Warren | Link | Lyons | Shivers | 4:34.85 | 2026 Falcon Friday |
| 4 | PALATKA |
A | Dean | Smith | Hammond | Oxendine | 3:59.02 | HBCU Relays Experience - High School Preview |
| 5 | PALATKA |
B | Fields | Woods | Passmore | Williams | NT | HBCU Relays Experience - High School Preview |
| 6 | FLORIDA DEAF AND BLIND |
A | Freeman | Vilsaint | Guzman | Logsdon | NT | Palatka Home Meet 2 |
| 7 | MENENDEZ |
B | Cardin | Lafron | Lor | Russell | NT |