| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | WILKINSON JUNIOR HIGH | A | Jeffries | Martin | Byers | Towne | NT | |
| 4 | ORANGE PARK JUNIOR HIGH | A | Vaughn | Duncan | Jones | Keeling | 4:50.00 | |
| 5 | GREEN COVE SPRINGS JUNIOR HIGH | A | 5:06.54 | Lake Asbury Quad Meet #1 | ||||
| 6 | GREEN COVE SPRINGS JUNIOR HIGH | B | NT |