| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | Matos, Ariana | FR | Plainfield (Central) | NT | |
| 4 | Prabhakar, Lily | SO | Rockford Christian | 1:02.00 | |
| 5 | Robinson, Naudia | SO | Plainfield (Central) | NT | |
| 6 | Richardson, Jinnaea | FR | Yorkville | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Thomason, Mya | FR | Dixon | 1:02.00 | |
| 2 | Weber, Olivia | SR | Fairbury (Prairie Central) | 54.00 | |
| 3 | Dixon, Kyi | JR | Lansing (Thornton Fractional South) | NT | |
| 4 | Russelburg, Maddie | SO | Yorkville | 50.74 | |
| 5 | Lynch, Emily | JR | Dixon | 51.12 | |
| 6 | Ifft, Megan | SR | Fairbury (Prairie Central) | 53.00 | |
| 7 | Dixon, Tajah | JR | Lansing (Thornton Fractional South) | NT | |
| 8 | Putnam, Karah | SO | Rockford Christian | 1:02.00 |