| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | MULVENNA, Tess | LaGrange Mavericks | NT |
| 3 | FITZPATRICK, Deenah | Hounds & Foxes Track Club | 38.15 |
| 4 | EGGERDING, Jenna | LaGrange Mavericks | 36.33 |
| 5 | ROY, Sophia | Hounds & Foxes Track Club | 38.07 |
| 6 | GRECH, Abby | LaGrange Mavericks | NT |
| 7 | WHEELER, Audrey | LaGrange Mavericks | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BRUNDIDGE, Sara | Hounds & Foxes Track Club | 34.17 |
| 2 | MAUER, Olivia | Chicago Rabbits | 33.31 |
| 3 | LARSEN, Ruby | Unattached | 32.00 |
| 4 | RICE, Genevieve | Cook Track Club | 31.51 |
| 5 | KAMALU, Lindora | Hounds & Foxes Track Club | 31.77 |
| 6 | LAWRENCE, Kayla | Unattached | 33.11 |
| 7 | WAIGHT, Bridget | LaGrange Mavericks | 33.45 |
| 8 | CHILSON, Lola | LaGrange Mavericks | 35.38 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | FUENTES, Lucia | Sauganash Striders | 30.77 |
| 2 | HAMB, Jordan | Hounds & Foxes Track Club | 29.65 |
| 3 | STRAYER, Audra | LaGrange Mavericks | 28.30 |
| 4 | MENDOZA, Sophia | Unattached | 27.49 |
| 5 | CASANOVA, Karina | Unattached | 27.84 |
| 6 | HAYWOOD, Anaya | Hounds & Foxes Track Club | 28.66 |
| 7 | FRAZIER, Alexis | Unattached | 30.35 |
| 8 | REED, Danyelle | Hounds & Foxes Track Club | 31.39 |