| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Our Lady of Perpetual Help | B | Harris | Conroy | Kramer | Dimberio | 1:08.20 | Verified |
| 3 | St. Joan of Arc Academy | A | Schultz | Cominos | Karter | Guiden | 1:07.40 | Verified |
| 4 | St Josaphat Middle School | A | Gertzner | Steele | Frech | Zupko | 1:07.10 | Verified |
| 5 | St. Joan of Arc Academy | B | Julien | Cominos | Wrigley | Wrigley | 1:07.40 | Verified |
| 6 | Our Lady of Perpetual Help | A | Cruz | Comer | Vogt | Tirpak | 1:08.20 | Verified |
| 7 | Sacred Heart | C | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Sacred Heart | B | Barlow | Creed | Haas | Benzer | DNS | Verified |
| 2 | St. Athanasius School | A | Reardon | Robertson | Herman-Lopez | Madison | 1:03.10 | Verified |
| 3 | Chicago Latin Upper School | A | Larsen | Di Silvestro | King | Khim | 1:00.00 | Unverified: |
| 4 | Alphonsus Academy | A | Berhalter | Rhomberg | Fabro | Superson | DNS | Verified |
| 5 | Haven Middle School | A | Addison | Strickland | LEAR | Jackson | 59.96 | Verified |
| 6 | Queen of All Saints | A | Nugent | Fitzgerald | Briody | Abraham | 1:03.00 | Unverified: |
| 7 | Catherine Cook School | A | Moroney | Howe | Rice | Hodes | 1:04.60 | Verified |
| 8 | Sacred Heart | A | Agdubai | Anderson | Coan | Lowe | DNS | Verified |