| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Parisi, Andrew | SO | Franklin Park-Northlake (Leyden) | NT | |
| 2 | Ruiz, Maneul | SO | Franklin Park-Northlake (Leyden) | 27.54 | |
| 3 | Hauger, Jack | SO | Addison Trail | 25.30 | |
| 4 | Valenti, Anthony | SO | Addison Trail | 24.43 | |
| 5 | Holliday, Deandre | SO | Willowbrook | 24.67 | |
| 6 | Wilcox, Michael | SO | Fenton | 26.58 | |
| 7 | Sosa, Juan Carlos | FR | Fenton | 28.75 | |
| 8 | Jagiello, Jake | FR | Willowbrook | NT |