| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | J. Sterling Morton Township | A | Butler, Oshanti | Borum, Kennedy | Nava, Isabella | Bautista, Esmeralda | |
| 2 | Trinity (River Forest) | A | Jacobs, Mary | Parker, Linda | Halloran, Annabel | Larocco, Arianna | |
| 3 | Willowbrook | A | Weder, Lily | Sanchez, Elizabeth | Barrington, Madeline | Williams, Samantha | |
| 4 | Conant | A | Yu, Elisa | Miltz, Ava | Feng, Maria | Bieneman, Ryan | |
| 5 | Addison Trail | A | Cervantes, Maritza | Campos, Jade | Ahmed, Nimrah | Tiscareno, Mayra | |
| 6 | Hinsdale South | A | Pacasova, Nicole | Hobbs, Ashley | Cius, Victoria | Abu suwelem, Sarah |