| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Addison (A. Trail) | A | Umana | Castillo | Esparza | Lazzara | 1:04.90 | Unverified: |
| 2 | Franklin Park-Northlake (Leyden) | A | Kendic | Pitts | Nimer | Buttitta | 58.40 | Verified |
| 3 | Darien (Hinsdale South) | A | Garro | Joseph | Patel | Lewen | 56.44 | Unverified: |
| 4 | Downers Grove (South) | A | McShan | Rizzo | Lander | Sztafa | 54.10 | Unverified: |
| 5 | Villa Park (Willowbrook) | A | Capps | Yeazel | Young | Bullivant | 54.17 | Unverified: Sue Pariseau Girls Invitational |
| 6 | Maywood (Proviso East) | A | McFarland | Kimble | Flowers | Evans | 56.49 | Verified |
| 7 | Berwyn-Cicero (Morton) | A | Marrero | Torres | Nelson | Vazquez | 59.33 | Unverified: |