| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | ORLOF, Sophia | JR | Franklin Park-Northlake (Leyden) | NT | Unverified: |
| 3 | TISCARENO, Mayra | SR | Addison (A. Trail) | 30.83 | Verified |
| 4 | TECUANHUEHUE, Jackie | SO | Addison (A. Trail) | 29.25 | Verified |
| 5 | MENDIOLA-MANDUJANO, Vanessa | JR | Franklin Park-Northlake (Leyden) | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LLORT, Karen | JR | Berwyn-Cicero (Morton) | 28.91 | Verified |
| 2 | BANDZOUMOUNA, Olivia | JR | Downers Grove (South) | 28.84 | Verified |
| 3 | MORRIS, Molly | JR | Villa Park (Willowbrook) | 27.98 | Verified |
| 4 | PETROVIC, Katarina | JR | Darien (Hinsdale South) | 27.18 | Verified |
| 5 | ALANDY, Brianna | JR | Villa Park (Willowbrook) | 27.21 | Verified |
| 6 | JENKINS, Natreya | JR | Downers Grove (South) | 28.12 | Verified |
| 7 | LAWSON, Jordan | JR | Maywood (Proviso East) | 28.89 | Unverified: |
| 8 | MOODY, Amaya | SR | Berwyn-Cicero (Morton) | 29.22 | Verified |