| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Olympia Fields (Rich Central) | B | Cannon, Kourtlyn | Harrison, Kendra | Smolenski, Kyndal | White, Morgan | NT | |
| 3 | Frankfort (Lincoln-Way East) | B | Sciarini, Katie | Barnard, Sophia | Clemmons, Jailee | Mills, Ashley | 4:32.00 | |
| 4 | Thornwood | A | Williams, Danaja | Moses, Cheyenne | Holmes, India | Simmons, Arryona | 4:20.00 | |
| 5 | Frankfort (Lincoln-Way East) | A | Ajifolokun, Ibukun | Marse, Sydney | Newton, Grace | Wright, Taylor | 4:15.00 | |
| 6 | Olympia Fields (Rich Central) | A | Criswell, Zaria | Campbell, Jayla | Ollins, Miana | Roberts, Zena | 4:20.00 |