| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 4 | SPYTEK, Kylie | Hawthorn South | 33.20 | |
| 5 | DARLING, Lucy | Hawthorn South | 36.60 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | PAVLU, Zoe | Lake Zurich South | 32.01 | |
| 2 | HOLLENSTEIN, Isabella | Grayslake | 32.00 | |
| 3 | FLANNIGAN, Maddie | Matthews | 31.50 | |
| 4 | CRUZ, Breana | Matthews | 31.00 | |
| 5 | MORGAN, Laila | Hawthorn North | 31.00 | |
| 6 | CHRISTIANSON, Allie | Barrington-Station Campus | 31.70 | |
| 7 | ESQUIVAL, Sophia | Wauconda | 32.00 | |
| 8 | SAVAGE, Stella | Wauconda | 32.20 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | ROSENZWEIG, Sofia | Barrington-Station Campus | 30.70 | |
| 2 | PRELL, Nikki | Barrington-Prairie Campus | 30.30 | |
| 3 | AKALAONU, Leialoha | Grayslake | 29.30 | |
| 4 | KAUFFMAN, Rayna | Lake Zurich South | 28.20 | |
| 5 | HINES, Nia | Hawthorn North | 29.04 | |
| 6 | PRATT, Audrey | Lake Zurich North | 29.70 | |
| 7 | GREGOIRE, Michaella | Lake Zurich North | 30.50 | |
| 8 | PIOTROWSKI, Melanie | Barrington-Prairie Campus | 30.80 |