| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Oak Lawn (Community) | A | Cabral, Ken | Valencia, Joshua | Cabalona, Masato | Gallagher, Chris | 1:41.00 |
| 4 | Midlothian (Bremen) | A | Porter, Taveon | Fus, Daniel | Hamilton, Tim | Smith, Izaiah | 1:39.00 |
| 5 | Tinley Park | A | Dudek, Davion | Fernandez, Izaiah | Davis, Ryan | Kovalcik, Zack | 1:40.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Oak Forest | A | Albin, Seth | Ashogbon, Adam | Scarpetta, Evan | Williams, Elijah | 1:36.00 |
| 3 | Chicago (Brother Rice) | A | Iraci, Giacomo | Ray, Nehemiah | McCoy, Tyrhen | Matejko, Anthony | 1:34.80 |
| 4 | Olympia Fields (Rich Central) | A | Hart, Obadiah | Roebuck, Zach | Miller, Mehki | Tyler, Sean | 1:32.30 |
| 5 | Oak Lawn (Richards) | A | Bonds, Eric | Brown, Darrion | Whitaker, Tristian | Perry, Anthony | 1:32.72 |
| 6 | Kankakee (Sr.) | A | Clark, Mattias | Davis, Darelle | Smith, Claude | Spivery, Chatavion | 1:35.12 |
| 7 | Chicago (Marist) | A | Watkins, Amari | Turner, Keyon | Davis, Antoine | Houston, Kivonte | DQ |