| Lane | Team | Squad | Seed |
|---|---|---|---|
| 3 | Chicago (Juarez) | A | NT |
| 3 | Chicago (Juarez) | A | NT |
| 4 | Chicago (Kennedy) | A | 1:06.45 |
| 5 | Chicago (Washington) | A | 1:11.88 |
| 5 | Chicago (Washington) | A | 1:11.88 |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | Chicago (King) | B | NT |
| 2 | Chicago (Agricultural Science) | A | DQ |
| 3 | Johnson College Prep | A | 54.80 |
| 4 | Perspectives IIT Math and Science | A | 53.93 |
| 5 | Chicago (Perspectives Charter/Calumet) | A | 54.67 |
| 6 | South Shore Int'l Prep | A | DNF |
| 7 | Chicago (Sullivan) | A | DNS |