| Lane | Team | Squad | Seed |
|---|---|---|---|
| 3 | Chicago (Agricultural Science) | A | NT |
| 4 | Chicago (Kennedy) | A | 1:06.45 |
| 5 | Chicago (Washington) | A | 1:12.13 |
| 6 | Chicago (Little Village) | A | DQ |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | Excel Academy of Englewood | A | DNS |
| 2 | Chicago (Perspectives Charter/Calumet) | A | 56.47 |
| 3 | Chicago (King) | A | 56.00 |
| 4 | Chicago (Providence-St. Mel) | A | 53.49 |
| 5 | Perspectives IIT Math and Science | A | 54.87 |
| 6 | Johnson College Prep | A | 56.38 |
| 7 | Chicago (Fenger) | A | 57.39 |
| 8 | Chicago (CICS/Ellison) | A | 1:03.46 |