| Lane | Team | Squad | Seed | 
|---|---|---|---|
| 3 | Chicago (Kennedy) | A | 53.79 | 
| 4 | Chicago (Agricultural Science) | A | 48.26 | 
| 5 | Chicago (Washington) | A | 49.57 | 
| 6 | Johnson College Prep | A | NT | 
| Lane | Team | Squad | Seed | 
|---|---|---|---|
| 1 | University of Chicago Charter School | A | 47.56 | 
| 2 | Chicago (Little Village) | A | 46.54 | 
| 3 | Chicago (King) | A | DNF | 
| 4 | Perspectives IIT Math and Science | A | 43.00 | 
| 5 | South Shore Int'l Prep | A | 44.08 | 
| 6 | Chicago (Perspectives Charter/Calumet) | A | 45.33 | 
| 7 | Chicago (Fenger) | A | 47.07 | 
| 8 | Chicago (CICS/Ellison) | A | 47.82 |