| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Valle, Daisy | SO | Chicago (Lindblom) | NM | |
| 2 | Golden, Vernisha | SO | Chicago (Perspectives Charter/Calumet) | 23' 5" | |
| 3 | Juste, Tamara | FR | South Shore Int'l Prep | 25' 11" | |
| 4 | Goss, Adrianna | SO | Chicago (Clark) | 29' 5.50" | |
| 5 | Lasose, Karen | JR | Chicago (Clark) | 31' 0" | |
| 6 | Pryor, Kaylen | SO | South Shore Int'l Prep | 31' 4" | |
| 7 | Adevor, Etornam | SO | Chicago (Kenwood) | 32' 1" | |
| 8 | Jackson, Zhania | SR | Chicago (Kenwood) | 32' 9.25" | |
| 9 | King, Kelani | SO | Chicago (Lindblom) | 33' 4" | |
| 10 | Riggins, Shakayla | FR | Chicago (Kenwood) | 33' 6" | |
| 11 | Marshall, Jordan | SO | South Shore Int'l Prep | 34' 8.50" | |
| 12 | Harrison, Amia | SO | Chicago (Simeon) | 35' 3.50" | |
| 13 | Boyd, Jalyn | SR | Chicago (Kenwood) | 36' 2.50" | |
| 14 | Wiggins, Chloe | FR | Chicago (Kenwood) | NM |