| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Cooley, Tamarion | FR | Chicago (Ogden International) | NM | |
| 2 | Blease, Crystal | JR | Chicago (Uplift) | NM | |
| 3 | Campbell, Taylor | JR | Chicago (Amundsen) | NM | |
| 4 | Lewin, Ciara | SR | Chicago (Lake View) | NM | |
| 5 | Washington, Kaela | FR | Chicago (Ogden International) | NM | |
| 6 | Rodriguez, Ashley | SO | Chicago (Amundsen) | NM | |
| 7 | Crawford, Mikiara | SO | Chicago (Prosser) | 10' 3" | |
| 8 | Espinoza, Sophia | SR | Chicago (Lake View) | 10' 7" | |
| 9 | Padillo, Alondra | JR | ITW David Speer Academy | 11' 6.75" | |
| 10 | Miller, Meagan | JR | Chicago (De La Salle) | 13' 0" | |
| 11 | Wright, Joya | SR | Chicago (De La Salle) | 14' 8" | |
| 12 | Dubose, Eriana | SO | ITW David Speer Academy | 15' 8.25" |