| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | Ludston, Jackie | SO | Norridge (Ridgewood) | 22.50 | |
| 3 | Diaz, Bianca | Norridge (Ridgewood) | 21.90 | ||
| 4 | Ikedi, Chize | JR | Palos Heights (Chicago Christian) | 20.34 | |
| 5 | Miller, Claire | FR | Timothy Christian | 20.91 | |
| 6 | Gallman, Ellie | SO | Palos Heights (Chicago Christian) | 21.90 |