| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Del Pena, Angela | SR | Norridge (Ridgewood) | 1:10.10 | |
| 2 | Stoll, Rachel | SO | Timothy Christian | 1:07.00 | |
| 3 | Groot, Emma | FR | Lansing (Illiana Christian) | 1:06.70 | |
| 4 | Hoeksttra, Olivia | JR | Timothy Christian | 1:02.90 | |
| 5 | Matos, Aingel | Elmwood Park | 1:06.60 | ||
| 6 | Czerwiec, Diana | Elmwood Park | 1:06.80 | ||
| 7 | Falb, Rebecca | JR | Palos Heights (Chicago Christian) | 1:08.80 | |
| 8 | Boksa, Kalina | SR | Norridge (Ridgewood) | DNS |