| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Park View School | A | Andrews, Alina | Rivas, Arianna | Amin, Saffa | Applequist, Maja | 2:16.75 |
| 4 | Lincoln Hall | A | Curescu, Emma | Shah, Aashi | Husaini, Zaynab | Jimenez, Jade | 2:07.45 |
| 5 | Old Orchard Junior High | A | Basillo, Danielle | Wideman, Ericka | Pak, Charis | Engstrom, Ava | 2:15.12 |
| 6 | Fairview South | A | Bui, Bridgit | Checke, Ruby | Lannoye, Sophia | Dervisevic, Minela | 2:21.00 |