| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Chicago (Payton) | A | NT | |||||
| 2 | Chicago (Horizon/McKinley) | A | NT | |||||
| 3 | Hancock High School | A | Gallegos | Del Rio | Castillo | Juarez | 5:37.15 | Verified |
| 4 | Beacon Academy | A | Santiaguillo | Reber | Yerkes | Hay | 4:55.80 | Verified |
| 5 | Chicago University High School | A | Mubayi | Park | Turek | Molony | 5:01.68 | Verified |
| 6 | Hancock High School | B | Toro | Moreno | Garcia Ocon | Cruz | 5:37.15 | Verified |
| 7 | Chicago (Latin) | A | DNS | Unverified: | ||||
| 8 | Chicago (Payton) | B | NT |