| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | LING, hana | Chicago (Payton) | NT | ||
| 3 | MANN, Julia | JR | Chicago (Latin) | 1:05.10 | Verified |
| 4 | SEDLER, Chloe | SO | Chicago (Latin) | 53.68 | Verified |
| 5 | GROTTHUSS, Julia | FR | Chicago University High School | 1:00.00 | Unverified: |
| 6 | RODRIGUEZ, Ruby | SO | Little Village High School | NT | Unverified: |