| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Chicago (Horizon/McKinley) | A | NT | |||||
| 2 | Hancock High School | A | Mirek | Pineda | Mendujano | Corral | 4:41.65 | Verified |
| 4 | Whitney Young High School | A | 3:55.00 | Unverified: | ||||
| 5 | Chicago University High School | A | Montague | Pytel | Ferreyra | Richardson | 3:39.53 | Verified |
| 6 | Chicago (Latin) | A | 3:45.59 | Verified | ||||
| 7 | Harvey (Thornton) | A | 3:58.39 | Verified | ||||
| 8 | Hancock High School | B | Flores | Martinez | Leanos | Rojas | 4:41.65 | Verified |
| 9 | Little Village High School | A | NT | Unverified: |