| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Clay, Natalya | SO | Chicago (Noble/Muchin) | 2:50.00 | |
| 2 | Hernandez, Jacqueline | SO | Chicago (Noble/Muchin) | 2:50.00 | |
| 3 | Rhodes, Aryana | JR | The Noble Academy | NT | |
| 4 | Villagran, Michelle | FR | The Noble Academy | NT | |
| 5 | Flores, Maribel | FR | Chicago (Hancock) | NT | |
| 6 | Guzman, Monica | SO | Chicago (Hancock) | NT | |
| 7 | Sotelo, Brenda | FR | Chicago (Kelly) | NT | |
| 8 | Wu, Mie Chen | JR | Chicago (Kelly) | NT | |
| 9 | Lee, Taylor | FR | Chicago (C. Hope Academy) | NT | |
| 10 | Muzikowski, Lace | FR | Chicago (C. Hope Academy) | NT |