| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | JOHNSBURG HIGH SCHOOL | A | 46.29 | Verified | ||||
| 3 | RICHMOND-BURTON HIGH SCHOOL | A | Jacoby | Verdoni | Martens | Rockwell | 45.45 | Verified |
| 4 | WOODSTOCK NORTH HIGH SCHOOL | A | 44.62 | Verified | ||||
| 5 | WOODSTOCK HIGH SCHOOL | A | Maidment | Sandoval | Dunnett | Sternitzky | 45.44 | Verified |
| 6 | MARENGO HIGH SCHOOL | A | Mirtl | Holst | Lohff | Davis | 45.61 | Unverified: |