| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | CHICAGO (UNIVERSITY) | A | Ewing, Mikaela | Barnard, Jane | Stevanovich, Alex | Wilson, Adria | 2:06.40 |
| 4 | CHICAGO (LATIN) | A | Kubert, Abigail | Darcy, Erico | George, Quincy | Aparti, Amanda | 2:00.78 |
| 5 | WINNETKA (NORTH SHORE COUNTRY DAY) | A | Hagerty, Caroline | Rice, Caroline | Roszak, Anna | Green, Liliana | 2:04.93 |