| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | CHICAGO (UNIVERSITY) | A | Polite, Maximos | Garfinkel, Max | Ovsiew, Adrian | Lau, Daniel | 51.74 |
| 4 | WINNETKA (NORTH SHORE COUNTRY DAY) | A | Isasi, Sebastian | Keyt, Nathan | Levin, Ethan | Xing, Andrew | 47.78 |
| 5 | NORTHRIDGE PREP | A | Jihad, Porter | Holohan, Sean | Vasquez, Anthony | Nuevo, Adam | 48.00 |
| 6 | CHICAGO (LATIN) | A | Mohammed, Anwar | Lugo, Kainoa | Cahn, Asher | Reese Clausen, Noah | 53.62 |