| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 2 | CHARFOOS, Isabella | SO | CHICAGO (FRANCIS W. PARKER) | NT |
| 3 | VALLIATH, Maya | SR | WINNETKA (NORTH SHORE COUNTRY DAY) | 1:05.56 |
| 4 | O'TOOLE, Charlotte | SO | CHICAGO (LATIN) | 56.36 |
| 5 | NAPPO, Sophia | SR | CHICAGO (LATIN) | 57.01 |
| 6 | STEVANOVICH, Alex | SR | CHICAGO (UNIVERSITY) | 1:07.03 |