| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | BARNARD, Jane | FR | CHICAGO (UNIVERSITY) | 32.39 |
| 2 | TATE, Tristen | FR | CHICAGO (FRANCIS W. PARKER) | 30.32 |
| 3 | KUBERT, Abigail | SR | CHICAGO (LATIN) | 29.01 |
| 4 | DARCY, Erico | JR | CHICAGO (LATIN) | 28.47 |
| 5 | WILSON, Adria | JR | CHICAGO (UNIVERSITY) | 28.79 |
| 6 | HAGERTY, Caroline | SO | WINNETKA (NORTH SHORE COUNTRY DAY) | 30.03 |
| 7 | MARCHAN, Ari | JR | ELGIN (E. ACADEMY) | 31.02 |
| 8 | ROSZAK, Anna | SR | WINNETKA (NORTH SHORE COUNTRY DAY) | 32.72 |