| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Chicago (Francis W. Parker) | A | Shadle, Audrey | Jain, Simran | Collins, Ada | Evan-Cook, Bella | 10:59.00 | |
| 2 | Winnetka (North Shore Country Day) | A | Chandler, Margaret | Doyle, Julia | Humphrey, Jordan | Hagerty, Caroline | 11:19.34 | |
| 3 | Chicago (Latin) | A | Syftestad, Olivia | O'Toole, Charlotte | Jagasia, Anika | Silverman, Sarah | 11:22.54 | |
| 4 | Chicago (University) | A | Herrara, Hannah | Wild, Franzi | Glick, Viviana | Atluri, Aanika | NT |