| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Mihas, Marianne | FR | Chicago (Latin) | 4:55.68 | |
| 2 | Hardiman, Clare | SO | Chicago (Latin) | 5:34.00 | |
| 3 | Shadle, Audrey | JR | Chicago (Francis W. Parker) | 5:47.35 | |
| 4 | Wild, Franzi | SO | Chicago (University) | 5:54.89 | |
| 5 | Glick, Viviana | FR | Chicago (University) | 5:58.31 | |
| 6 | Evan-Cook, Bella | JR | Chicago (Francis W. Parker) | 6:04.49 | |
| 7 | Humphrey, Jordan | FR | Winnetka (North Shore Country Day) | 6:20.43 | |
| 8 | Kaniewski, Liza | SR | Chicago (Latin) | 6:24.22 | |
| 9 | Nisbet, Arleigh | JR | Elgin (E. Academy) | NT | |
| 10 | Jagasia, Anika | SR | Chicago (Latin) | NT |