| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | RYBARCZYK, Jameson | 0 | Illinois Lightning Track Club | NT | Unverified: |
| 3 | FRAUSTO, Cain | -- | Illinois Lightning Track Club | NT | Unverified: |
| 4 | MORGAN, Cameron | -- | Illinois Lightning Track Club | 46.00 | Unverified: |
| 5 | WILSON JR, Dion | -- | Illinois Lightning Track Club | 49.76 | Verified |
| 6 | RANGEL, Chase | -1 | Unattached | NT | Unverified: |
| 7 | SOSNOWSKI, Caleb | -- | Illinois Lightning Track Club | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | RODRIGUEZ, Jahaziel | -- | Illinois Lightning Track Club | 45.00 | Unverified: |
| 3 | GHOLSTON, Dallas | -- | Illinois Lightning Track Club | 43.37 | Verified |
| 4 | SHAKESPEARE, Breylen | 8 | Rush Athletics USA | 36.37 | Verified |
| 5 | PORTER, Luke | -- | Illinois Lightning Track Club | 43.00 | Unverified: |
| 6 | LUDWIG, Will | -- | Illinois Lightning Track Club | 44.00 | Unverified: |
| 7 | GHOLSTON, Derrick | -- | Illinois Lightning Track Club | 45.60 | Verified |