| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MCFADDEN, Carmelo | Wildcat Track Club | NT | ||
| 3 | O'SHIELDS, Mason | -- | University of Chicago TC | 28.31 | Verified |
| 4 | TOLBERT, Derrick | -- | Rush Athletics USA | 25.67 | Verified |
| 5 | SMITH, Kadin | 8 | University of Chicago TC | 25.81 | Verified |
| 6 | MEANS, Tyler | SO | University of Chicago TC | NT | Unverified: |
| 7 | SELLERS, Kendrick | SR | University of Chicago TC | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | JOHNSON, Conner | 9 | Chicago Thunder Track Club | 25.23 | Verified |
| 3 | MYERS, Troy | SO | Unattached | 24.30 | Verified |
| 4 | TERRY, Rodney | SO | Illinois Lightning Track Club | 23.66 | Unverified: |
| 5 | LANSDOWNE, Jake | JR | Unattached | 23.85 | Verified |
| 6 | HOUSE JR, ANTHONY | -- | Rush Athletics USA | 24.80 | Unverified: |
| 7 | ST CLAIR, Malik | -- | University of Chicago TC | 25.43 | Verified |