| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | Mason Meister | FR | Lafayette Central Catholic | 25.90 |
| 2 | Mark Kube | FR | Eastern Hancock | 24.83 |
| 3 | Myreon DeVost | SO | Western | 24.59 |
| 4 | Jaylyn Pugh | JR | Hamilton Heights | 24.58 |
| 5 | Conor Baumann | JR | Hamilton Heights | 24.59 |
| 6 | Dylan Bowman | JR | Eastern Hancock | NT |
| 7 | Nate Wetherell | JR | Seton Catholic | 25.24 |
| 8 | Aaron Lee | JR | Greenfield-Central | NT |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | Ryan Murray | SR | Seton Catholic | 24.15 |
| 2 | Dillon Pratt | JR | Frankton | 23.88 |
| 3 | Kirk Knecht | JR | Greenfield-Central | 23.55 |
| 4 | Luke Moody | SR | Guerin Catholic | 23.09 |
| 5 | Noah Smith | FR | Lafayette Central Catholic | 23.49 |
| 6 | Alex Gibbs | JR | Guerin Catholic | 23.65 |
| 7 | Simon Aaron | SR | Western | 24.12 |
| 8 | Austin Nunley | JR | Frankton | 24.16 |