| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Bartlett | A | Burnett-Stokes | Krysko | Jasso | Chin | 49.82 | Unverified: |
| 4 | Wheaton (North) | A | 48.20 | Unverified: | ||||
| 5 | Glen Ellyn (Glenbard South) | A | 49.72 | Unverified: | ||||
| 6 | Roselle (Lake Park) | A | Matsuyama | Tourtillot | Lanzito | Szkaradek | 50.14 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Niles West High School | A | Cotofrea | Delisi | Werth | Gashew | 47.51 | Unverified: |
| 2 | Naperville (North) | A | Neubert | Gehrs | Westlake | Duckworth | 47.00 | Unverified: |
| 3 | Lowell High School | A | Rosenthal | Sanchez | James | Harmston | 46.32 | Verified |
| 4 | Glen Ellyn (Glenbard West) | A | Vargas | Benson | Ellens | Thompson | 46.00 | Unverified: |
| 5 | Evanston (Twp.) | A | Schwartz | Timberlake | Gumbs | Jean-Francois | 46.22 | Unverified: |
| 6 | Oak Park (O.P.-River Forest) | A | Markley | Jones | Fitzpatrick | Nichols | 46.54 | Unverified: Bob Cohoon Invitational |
| 7 | Berwyn-Cicero (Morton) | A | Ayala | Dunbar | Torres | Gonzalez | 47.19 | Verified |
| 8 | South Elgin | A | Crea | Velazquez | Pruitt | Norowol | 48.04 | Verified |