| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Ogden International | A | NT | ||||
| 2 | Oak Lawn Community High School | A | Torres | Quinones | Canales | Sheridan | 4:42.26 |
| 3 | Fenton High School | A | Castillo | Durkowski | Martens | Miller | 4:38.49 |
| 4 | Grayslake Central High School | A | Sato | Morello | Guckenberger | Domier | 4:18.43 |
| 5 | Hersey High School | A | Hany | Makowski | Bialko | Engle | 4:19.32 |
| 6 | Montini Catholic | A | Kehoskie | Juda | Gergen | Fisher | 4:40.00 |
| 7 | Glenbard South High School | A | Garcia | Izaguirre | Winter | Zeman | 4:46.27 |
| 8 | Elgin High School | A | 5:26.40 |