| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Ogden International | A | DNS | ||||
| 3 | Elgin High School | A | 1:06.24 | ||||
| 4 | Grayslake Central High School | A | Desai | Magliola | Stumbo | Spencer | DQ |
| 5 | Hersey High School | A | Ilinykh | Dubon | Settersten | Shah | 55.80 |
| 6 | Glenbard South High School | A | Flores | Frieling | Peshtani | Hernandez | 1:07.22 |
| 7 | Streamwood High School | A | Navarro | Iachim | Michell | Pena | NT |