| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 1 | Bleakley, Amy | SO | Batavia | 28.33 | 
| 2 | Macabobby, Grace | FR | Wheaton Warrenville South | 27.89 | 
| 3 | Capel, Arielle | JR | Carol Stream (Glenbard North) | 27.46 | 
| 4 | McNamara, Katie | JR | Geneva | 27.14 | 
| 5 | Lucer, Paulina | FR | Roselle (Lake Park) | 27.37 | 
| 6 | Frederick, Katherine | SR | St. Charles (East) | 27.57 | 
| 7 | Milnamow, Claire | SR | Wheaton Warrenville South | 27.94 | 
| 8 | Ozeki, Sarah | SO | Carol Stream (Glenbard North) | NT | 
| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 1 | Sanchez, Vanessa | SO | Roselle (Lake Park) | 26.93 | 
| 2 | Macris, Christie | SR | Wheaton (North) | 26.83 | 
| 3 | Hopp, Kaylee | SO | St. Charles (North) | 26.64 | 
| 4 | Zander, Leeya | JR | Wheaton (North) | 25.67 | 
| 5 | Siciliano, Michelle | SR | Geneva | 26.46 | 
| 6 | Wilson, Davinia | SO | Batavia | 26.69 | 
| 7 | Custer, Nikole | JR | St. Charles (North) | 26.84 | 
| 8 | Brown, Makenna | SO | St. Charles (East) | 26.98 |