| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Sosa, Juan Carlos | FR | Fenton | 1:02.46 | |
| 2 | Ansari, Omar | SR | Hoffman Estates | 56.52 | |
| 3 | Panozzo, Zach | SO | Lombard (Glenbard East) | 56.07 | |
| 4 | Horvath, Will | SO | Mt. Prospect (Prospect) | 53.92 | |
| 5 | Nowaczyk, Kacper | SO | Franklin Park-Northlake (Leyden) | 55.38 | |
| 6 | Lebourhis, Tristan | SO | Maine South | 56.50 | |
| 7 | Monaco, Jorge | FR | Schaumburg | 57.68 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Rodriguez, Angel | JR | Addison Trail | 53.57 | |
| 2 | Block, Dylan | JR | Conant | 53.10 | |
| 3 | Issac, Oneil | SO | Maine East | 52.20 | |
| 4 | Arroyo, Aaron | SR | Minooka | 50.29 | |
| 5 | William, Kevon | SR | Proviso West | 51.50 | |
| 6 | Hughes, Kentae | FR | Zion-Benton | 52.40 | |
| 7 | Rogus, Kyle | SR | Glenbard West | 53.50 | |
| 8 | Gay, Jalil | SR | Rock Island | 53.67 |