| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MILLER, Anna | Hoffman Estates | 2:16.90 | |
| 2 | LUTZOW, Jenna | Belvidere (North) | 2:18.61 | |
| 3 | WIEDACHER, Jenna | Lombard (Montini) | 2:22.07 | |
| 4 | LEENEY, Molly | Mt. Prospect (Prospect) | 2:24.80 | |
| 5 | WARTMAN, Kayla | Conant | 2:25.24 | |
| 6 | SHELTON, Emily | Minooka | 2:26.32 | |
| 7 | NAVARRETE, Veronica | Morton | 2:28.74 | |
| 8 | MENDOZA, Jacqueline | Chicago (Taft) | 2:34.41 | |
| 9 | SZKARADEK, Kasia | Roselle (Lake Park) | 2:34.62 | |
| 10 | BETLEJESKI, Isabella | Joliet (West) | 2:35.00 | |
| 11 | KACZMARZ, Nadia | Riverside (R.-Brookfield) | 2:41.35 | |
| 12 | KELLY, Morgan | Bartlett | 2:44.00 | |
| 13 | CONFER, Maddy | Rockford (Guilford) | 2:50.00 |